In this study the concentrations of Cu, Fe, Zn, Cd, Pb and Ni were determined in muscle tissue and liver of three fish species (Mugil liza – mullet, illiophagus; Micropogonias furnieri – croaker, carnivorous, and Trichiurus lepturus – cutlassfish, piscivore), in the muscle tissue of a shrimp species (Farfantepenaeus brasiliensis – pink shrimp, omnivore) and in muscle tissue, entrails and eggs of a soft crab (Callinectes spp. – siri azul, omnivore) sampled from the Ilha Grande Bay. The concentrations of the elements were determined by inductively coupled plasma mass spectrometry (ICP-MS). The methodology showed good accuracy and precision when tested with certified reference materials (DORM-3 and TORT-2). The concentrations of Cu, Zn, Cd, Ni and Pb were below the limits established by the Brazilian and American legislation for human ingestion of fish products. The highest concentrations of Cu, Fe, Zn, Pb, Cd and Ni were found in the crustacean samples. In fish, the highest concentrations were observed in the liver and, in the soft crab samples, in the entrails. Sex did not influence element accumulation in fish, but influenced the accumulation of Cu, Fe, Zn, Cd and Ni in the soft crab. In fish, most elements did not show any correlation to length and weight, with the exception of Pb, which showed negative correlations in mullet and croaker and Cd which showed negative correlations in croaker. In crustaceans, some elements also presented negative correlations to these parameters, indicating that both in fish and crustaceans these elements are not accumulated during organism growth. The concentrations of the elements analyzed were statistically different in the dry and rainy seasons.