The São Pedro da Serra Region has two types of tillage for the sowing soil, the shifting cultivation is based on the soil area rotation and the traditional utilize high amount of agro-toxics products. The aim of this work is to study the 2,4 D herbicide fixation and mobilization from the soil characteristics and the tillage. The cultivated areas were divided depending of the sow time as: system 1 (shifting cultivation 4-7 years), system 2 (shifting cultivation 10-12 years), system 3 (traditional sow), system 4 (forest area). Three different depths were selected (0-10 cm, 10-20 cm, 25-40 cm). The four different systems were compared using the mineralogical characterization, the soil micro morphology, the physic and chemic fractionation for the organic material and the herbicide quantification by the HPLC technique. The semi quantitative results using the HPLC verify the 2,4- D herbicide contains for all the studied systems. The 2,4-D concentration have correlation with the amount of clay of the soil, and have correlation negative with the amount of Al3+ and H+. The relation depends on the herbicide and soil chemical characteristics. The study found that the shifting cultivation had the best 2,4-D elimination, The shifting cultivation soil management permit the better soil regeneration of the chemical and physical properties and the agro-toxic use reduction and the hydro source contamination.