Ecosystem services (ES) are the benefits that society obtains from ecosystems and are fundamental to human well-being. For this framework to be used in decision making, the first step is mapping ES. This study mapped the provision of eight ES for the city of Rio de Janeiro - flood control, air quality maintenance, climate regulation, mitigation of storms and extreme events, scenic beauty, religion and spirituality, recreation and tourism, and physical and mental health. Mapping was accomplished through reclassification of land use and land cover data, definition of priority ESs, and interviews with experts. The study found that there was an increase in the percentage of urban area and a decrease in the provision of five SEs in all regions of the city. The study also mapped the supply and demand of cultural ecosystem services (CES), to identifying the relationship of the city s inhabitants with local ecosystems through the application of an electronic questionnaire. It was found that the inhabitants of the South Zone and West Zone preferably frequent areas close to the neighborhoods and, as a priority, the beaches. The North Zone is the region with the highest percentage of urban area (82 percent) and the lowest offer of natural areas. Although the city has an enormous diversity of ecosystems, these are not equally distributed in the territory and there are significant differences both in the supply of natural areas and in socioeconomic characteristics when we compare the five large municipal Planning Areas.